Resealable plastic sandwich bag Small glass jar such as a spice or baby food jar Bamboo skewer, available at most grocery stores. If you use a baby food or short spice jar, you could substitute a toothpick for the skewer.
Put strawberry into freezer baggie Mash for 2 minutes Add 10 ml DNA extraction solution into baggie Gently mix strawberry mash solution for 1 minute Cool baggie in ice bath to precipitate proteins Filter mashed solution through filter into clear plastic cup that is in ice bath if using ethanol.
Allow filtered DNA solution to cool a minute. Carefully pour alcohol along side of cup. Use 2x the volume alcohol as DNA solution. Let the two layers of liquid settle 3 minutes. Background DNA is abundant in strawberries.
For this reason, it is easy to extract DNA from strawberries. Strawberry cells each contains 8 sets of chromosomes. What was the purpose of mashing up the strawberry? To break down the cell wall, cellular and nuclear membranes. Where is DNA found in strawberry cells?
What is the purpose of detergent in the extraction solution? Soap dissolves cell membranes so that DNA is liberated. What is the purpose of EDTA in the extraction solution? Why is the solution filtered? This filters out larger particles, such as cell walls, etc.
What happened when alcohol was added to the solution? DNA is negatively charged and remains soluble in water. Alcohol contains non-polar non-charged molecules.
This is called a precipitate. If DNA is so small, how come we can see it? Many threads of DNA bundle together so that we can see it.SWBAT utilize everyday materials to extract and view DNA from a strawberry. Big Idea This lesson provides students with the chance to practice proper laboratory procedures while extracting DNA from strawberries.
DNA Isolation from Strawberries Student Directions Materials per student group • strawberries (about the volume of a golf ball). Frozen strawberries should be thawed at room temperature.
• 10 ml DNA Extraction Buffer (soapy salty water) • about 20 ml ice cold 91% or % isopropyl alcohol • 1 Ziploc TM bag • 1 clear test tube.
DNA is the blueprint for life. This simple experiment will show you how to extract DNA from fruit like a banana or strawberry. All you need are some fruit and some .
The DNA can be used for a variety of uses after extraction.
you can use polymerase chain reaction (PCR) to amplify certain regions of the plant's genome to look for various genetic markers (such as to identify the breed of strawberry). The procedures to extract DNA from a strawberry are relatively similar to advanced laboratory DNA extraction procedures.
Both require a mechanical disruption of cells, liberation of the DNA, and precipitation of the DNA. Extracting DNA from Strawberries: A super fun experiment we found on KidScienceApp is extracting DNA from strawberries.
DNA is the blueprint for all organisms. It can be found in the nucleus (via The Homeschool Scientist) [ ].